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One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.

Genetic engineering. --- Human genetics. --- Animal genetics. --- Transgenic organisms. --- Genetic Engineering. --- Human Genetics. --- Animal Genetics and Genomics. --- Transgenics. --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Organisms --- Genetic engineering --- Genetics --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Heredity, Human --- Human biology --- Physical anthropology

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Recent scientific advances have made it possible to produce biopharmaceuticals in genetically modified plants and animals, such as maize, tobacco, goats, and chickens. This new branch of biotechnology is termed pharming, composed of the terms pharmaceuticals and farming. Pharming constitutes an overlap of red and green biotechnology. It offers the prospect of a quicker, cheaper, and more flexible production of biopharmaceuticals compared with current production processes. This is a promising perspective in light of the rapidly growing market of biopharmaceuticals, although the economic competitiveness of pharming remains to be proven. Besides possible benefits for producers, patients and health care systems, pharming also raises a number of complex ecological, social, moral and legal questions that have as yet not been thoroughly discussed. The present book contains the findings of an interdisciplinary research project that has addressed a large range of questions associated with pharming: An analysis of the state-of-the-art of plant pharming and animal pharming technologies is followed by an assessment of environmental risks related to pharming and welfare risks for pharming animals. Public views and attitudes to pharming are investigated on the basis of a comprehensive survey in 15 countries. Moreover, ethical and legal questions, posed by present and foreseeable future practices of pharming, are analysed. The concluding chapter presents the authors' main findings and recommendations, addressed to science, industry, politics and general public interested in the chances and risks of this upcoming field of biotechnology.

General ethics --- Human biochemistry --- Pharmacology. Therapy --- Biochemical engineering --- medische biochemie --- bio-engineering --- farmacie --- farmacologie --- biogeneesmiddelen --- biochemie --- ethiek --- toxicologie --- Biopharmaceutics --- Genetic engineering --- Pharmaceutical biotechnology --- Biopharmaceutical technology --- Drugs --- Biotechnology --- Pharmaceutical technology --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Transgenic organisms --- Pharmacology --- Pharmacy

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Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

Cell lines. --- Cell culture. --- Cultures (Biology) --- Cytology --- Cell culture --- Technique --- Medicine. --- Cytology. --- Genetic engineering. --- Biochemistry. --- Biomedicine general. --- Cell Biology. --- Genetic Engineering. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Composition --- Cell biology. --- Biomedicine, general. --- Health Workforce --- Medicine --- Biotechnology. --- Biomedical Research. --- Chemical Bioengineering. --- Research. --- Chemical engineering --- Genetic engineering --- Biological research --- Biomedical research

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Louis-Marie Houdebine and Jianglin Fan The study of biological functions of proteins and their possible roles in the pathogenesis of human diseases requires more and more relevant animal m- els. Although mice including genetically modified mice offer many possibilities, other non-murine species are absolutely required in some circumstances. Rabbit is one of these species, which has been widely used in biomedical studies. This animal is genetically and physiologically closer to humans including cardiov- cular system and metabolism characteristics. Rabbit is thus more appropriate than mice to study some diseases such as atherosclerosis and lipid metabolism. Because of its larger size, surgery manipulation, bleeding, and turn-over studies are much easier performed in rabbits than in mice. Furthermore, transgenic rabbits can be produced using microinjection and other methods such as lentiviral v- tors. Cloning in rabbits has been proved possible, even though still laborious and time-consuming. Hopefully, functional rabbit ES cell lines will be available in the coming years. Gene deletion or knock-out in rabbits will then become possible.

Animal genetic engineering. --- Rabbits as laboratory animals. --- Rabbits --Genetics. --- Transgenic animals. --- Transgenic animals --- Rabbits --- Animal genetic engineering --- Rabbits as laboratory animals --- Animals, Genetically Modified --- Animals, Laboratory --- Genetic Engineering --- Genetics --- Lagomorpha --- Genetic Techniques --- Animal Population Groups --- Organisms, Genetically Modified --- Biology --- Animals --- Biological Science Disciplines --- Organism Forms --- Investigative Techniques --- Mammals --- Eukaryota --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Vertebrates --- Natural Science Disciplines --- Organisms --- Disciplines and Occupations --- Chordata --- Health & Biological Sciences --- Breeding. --- Physiology. --- Rabbit breeding --- Medicine. --- Biotechnology. --- Genetic engineering. --- Animal genetics. --- Biomedicine. --- Biomedicine general. --- Genetic Engineering. --- Animal Genetics and Genomics. --- Laboratory animals --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Chemical engineering --- Genetic engineering --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Health Workforce --- Biomedicine, general.

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To what extent should we use technology to try to make better human beings? Because of the remarkable advances in biomedical science, we must now find an answer to this question. Human enhancement aims to increase human capacities above normal levels. Many forms of human enhancement are already in use. Many students and academics take cognition enhancing drugs to get a competitive edge. Some top athletes boost their performance with legal and illegal substances. Many an office worker begins each day with a dose of caffeine. This is only the beginning. As science and technology advance further, it will become increasingly possible to enhance basic human capacities to increase or modulate cognition, mood, personality, and physical performance, and to control the biological processes underlying normal aging. Some have suggested that such advances would take us beyond the bounds of human nature. These trends, and these dramatic prospects, raise profound ethical questions. They have generated intense public debate and have become a central topic of discussion within practical ethics.Should we side with bioconservatives, and forgo the use of any biomedical interventions aimed at enhancing human capacities?

Biomedical Enhancement --- Genetic Engineering --- Genetic engineering. --- Medical ethics. --- Medical innovations --- Prenatal Diagnosis --- Prenatal diagnosis. --- ethics. --- Social aspects. --- Diagnostics prénatals --- Prenatal diagnosis --- genetische verbetering (maakbare mens) --- amélioration génétique --- Diagnostics prénatals --- Genetic engineering --- Medical ethics --- bio-ethiek (medische, biomedische ethiek, bio-ethische aspecten) --- verbetergeneeskunde (mensverbetering) --- Antenatal diagnosis --- Intrauterine diagnosis --- Prenatal testing --- Diagnosis --- Obstetrics --- Society and medical innovations --- Biomedical ethics --- Clinical ethics --- Ethics, Medical --- Health care ethics --- Medical care --- Medicine --- Bioethics --- Professional ethics --- Nursing ethics --- Social medicine --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Social aspects --- bioéthique (éthique médicale, biomédicale, aspects bioéthiques) --- médecine de l'amélioration (médecine d'amélioration) --- Moral and ethical aspects --- Professional ethics. Deontology --- medische ethiek --- Ethics. --- Medecine --- Ethique médicale --- Génie génétique --- Innovations --- Aspect social --- Medical innovations - Social aspects